| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ye, Brandon Z (15)
|
MAGRA |
50.40 |
49.98 |
-0.42 |
| 2 |
Naessig, Christopher D (16)
|
MAPVAC |
50.64 |
52.73 |
2.09 |
| 3 |
Gorman, Ronan M (17)
|
MAGRA |
51.63 |
53.00 |
1.37 |
| 4 |
March, Oliver R (16)
|
MAGRA |
53.62 |
53.85 |
0.23 |
| 5 |
Krein, Christopher A (16)
|
MAMSA |
53.29 |
54.26 |
0.97 |
| 6 |
Tebo, Tyler V (15)
|
MAGRA |
54.87 |
55.57 |
0.70 |
| 7 |
Thompson, Will C (15)
|
MAMSA |
55.47 |
56.09 |
0.62 |
| 8 |
Silverstein, Luke J (15)
|
MAGRA |
55.11 |
56.24 |
1.13 |
| 9 |
DeHaven, Regan J (15)
|
MAWA |
57.10 |
56.65 |
-0.45 |
| 10 |
Orsulak, Michael R (15)
|
MAMSA |
56.16 |
57.20 |
1.04 |
| 11 |
Pennington, Ryder L (17)
|
MACAA |
54.55 |
57.77 |
3.22 |
| 12 |
Grogan, Tatum J (15)
|
MAMSA |
58.01 |
58.62 |
0.61 |
| 13 |
Rasmussen, Alexander B (16)
|
MAFWAC |
1:02.50 |
1:01.39 |
-1.11 |
| 14 |
Grainda, Brady M (15)
|
MAMSA |
1:02.95 |
1:01.90 |
-1.05 |
|
Zabigalivk, Maksym (15)
|
MANSC |
59.08 |
NS |
- |
|
Hickey, Andrew (17)
|
MAWCAY |
55.70 |
NS |
- |